Department of Chemistry & Biochemistry
2024 Spring Workshop on
Protein Structure Elucidation with Ion Mobility
Spectrometry/Mass Spectrometry
Experimental methods: TIMS/MS and Tandem-TIMS/MS for Native Mass Spectrometry and Top-Down Protein Analysis
Computational methods: Projection Superposition Approximation (PSA) and Structure Relaxation Approximation (SRA)
Spring Workshop Content
All lectures and lab courses will be held at the Department of Chemistry and Biochemistry, Florida State University.
2024 Spring Workshop on Protein Structure Elucidation with Ion Mobility Spectrometry/Mass Spectrometry
Bleiholder Laboratory
Sunday, March 10
| Time | Activity |
| 6.00-8.00 pm | Meet & greet |
Monday, March 11
| Time | Lecture Title |
| 9:00-9:20 | Introduction to workshop & overview of lecture contents (Bleiholder) |
| 9:20-9:45 | Introduction to ion mobility spectrometry (Bleiholder) |
| 10:00-10:45 | Proteins in the gas phase: Native ion mobility/mass spectrometry (Liu) |
| 11:00-11:45 | TIMS/MS, Tandem ion mobility spectrometry & tandem-TIMS/MS (Liu) |
| Lunch break | |
| 1:15-3:00 | Tutorial I. TIMS/MS and Tandem-TIMS/MS for Native MS (Liu) |
| 3:15-5:00 | Tutorial II. TIMS/MS and Tandem-TIMS/MS for Top-Down Protein Analysis (Liu) |
| Dinner |
Tuesday, March 12
| Time | Lecture Title |
| 9:00-9:45 | Invited guest speaker |
| 10:00-10:45 | Fundamentals of ion mobility spectrometry (Bleiholder) |
| 11:00-11:45 | Projection Superposition Approximation (PSA) (Bleiholder) |
| Lunch break | |
| 1:15-3:00 | Tutorial III. Projection Superposition Approximation (PSA) (Bleiholder) |
| 3:15-5:00 | Tutorial IV. Projection Superposition Approximation (PSA) (Bleiholder) |
| Dinner |
Wednesday, March 13
| Time | Lecture Title |
| 9:00-9:45 | Structure Relaxation Approximation (SRA) (Bleiholder) |
| 10:00-10:45 | Tutorial V. Structure Relaxation Approximation (SRA) (Bleiholder) |
| 11:00-11:45 | Q&A |
| 11.45-1.30 | Lunch & adjourn |
Literature:
TIMS/MS and Tandem-TIMS/MS:
- Liu, F. C., Kirk, S. R., & Bleiholder, C. (2016). On the Structural Denaturation of Biological Analytes in Trapped Ion Mobility Spectrometry – Mass Spectrometry. Analyst 141, 3722-3730. (Special Issue: 2016 Emerging Investigators).
- Liu, F. C., Ridgeway, M. E., Park, M. A., Bleiholder, C. (2018). Tandem trapped ion mobility spectrometry. Analyst 143 (10), 2249-2258.
- Liu, F. C., Ridgeway, M E., Park, M. A., & Bleiholder, C. (2022). Tandem-trapped ion mobility spectrometry/mass spectrometry (tTIMS/MS): a promising analytical method for investigating heterogenous samples . Analyst 147, 2317-2337.
PSA
- Bleiholder, C., Wyttenbach, T., & Bowers, M. T. (2011).A novel projection approximation algorithm for the fast and accurate computation of molecular collision cross sections (I). Method. International Journal of Mass Spectrometry 308, 1-10.
- Anderson, S. E., Bleiholder, C., Brocker, E. R., Stang, P. J., & Bowers, M. T. (2012). A novel projection approximation algorithm for the fast and accurate computation of molecular collision cross sections (III). Application to Supramolecular Coordination-Driven Assemblies. International Journal of Mass Spectrometry 330-332, 78-84. (Armentrout Honor Issue).
SRA
- Bleiholder, C., & Liu, F. C. (2019). Structure Relaxation Approximation (SRA) for Elucidation of Protein Structures from Ion Mobility Measurements. Journal of Physical Chemistry B 123 (13), 2756-2769.
- Cropley, T. C., Liu, F. C., Pedrete, T., Hossain, MD A., Agar, J. N., & Bleiholder, C. (2023). Structure Relaxation Approximation (SRA) for Elucidation of Protein Structures from Ion Mobility Measurements (II). Protein complexes . Journal of Physical Chemistry B 127 (25), 5553–5565. (Special Issue: Early-Career and Emerging Researchers in Physical Chemistry Volume 2).
