Answer:
Spot A: Aspartic Acid
Rf = (7.0 cm - 1.5 cm) / (5.35 cm - 1.5 cm) = 0.7
Spot B: Asparagine
Rf = (7.0 cm - 1.5 cm) / (4.8 cm - 1.5 cm) = 0.6
| Example 1: Identify the amino acid in a chromatogram by calculating their Rf values. Answer: Spot A: Aspartic Acid Rf = (7.0 cm - 1.5 cm) / (5.35 cm - 1.5 cm) = 0.7 Spot B: Asparagine Rf = (7.0 cm - 1.5 cm) / (4.8 cm - 1.5 cm) = 0.6 |