Lab Procedure

SAFETY NOTES: The alcohol used for this experiment is denatured and should not be consumed. It will make you very ill! The organic solvents should be disposed of in the hood immediately after use. Do not inhale the fumes!

GENERAL INSTRUCTIONS: Each student will work alone for this experiment

Part 1: Density of ethanol
	Obtain the weight of your 10mL graduate cylinder to the nearest 0.0001g and record in your notebook.
	Obtain approximately 3.0 mL of Pure Ethanol in your graduated cylinder, record the volume of ethanol to the nearest 0.01mL.
	Reweigh the graduated cylinder containing ethanol to the nearest 0.001g
Part II: Density of Water
	Obtain approximately 3.0 mL of D.I. Water in your previously weighed graduated cylinder, record the volume of water to the nearest 0.01mL.
	Reweigh the graduated cylinder containing water to the nearest 0.0001g
Part III: Volume percent of alcohol
	Obtain the weight of your 25mL graduated cylinder to the nearest 0.0001g and record in your notebook.
	Add 25mL of unknown to your pre-weighed graduated cylinder, touching the graduated cylinder only with a kimwipe. Record the exact volume of unknown to the nearest 0.01mL. Weigh and record the mass of the graduated cylinder with unknown to the nearest 0.0001g.
	Carefully add the 25mL of unknown to your sepratory funnel; make sure the stop cock is CLOSED. Try to get as much of the liquid into the funnel as possible.
	Using a weighboat carefully weigh out 1.0 g of NaCl. Record the exact mass in your lab notebook.
	Add the salt to the separatory funnel and shake until the salt completely dissolves. Vent the funnel as demonstrated by your TA about every 15 seconds. MAKE SURE THE FUNNEL IS POINTING AWAY FROM OTHER STUDENTS AND YOURSELF.
	Add 10mL of ethyl acetate (located in the hood) to your sepratory funnel. Cap funnel and shake and then vent, as shown by your TA prior to performing the experiment. Replace cap and shake vigorously, venting periodically. MAKE SURE THE FUNNEL IS POINTING AWAY FROM OTHER STUDENTS AND YOURSELF.
	Add 10mL of diethyl ether (located in the hood) to your sepratory funnel. Cap funnel and shake and then vent, as shown by your TA prior to performing the experiment. Replace cap and shake vigorously, venting periodically. MAKE SURE THE FUNNEL IS POINTING AWAY FROM OTHER STUDENTS AND YOURSELF. Set the separatory funnel in a 400 mL beaker and allow the polar and non-polar layers to separate. Two distinct layers should be visible.
	Remove cap, and turn stop cock to slowly and carefully drain the bottom yellow layer from your funnel into your pre-weighed 25mL graduated cylinder. Try to get all of the yellow later out of the funnel without losing any of the clear organic layer.
	Dispose of the clear organic layer in the waste jar provided in the hood.
	Record the volume and weight of the yellow liquid in your 25mL graduated cylinder.
	Dispose of the yellow liquid down the sink drain. Clean the graduated cylinder and separatory funnel thoroughly. Wash your hands well before leaving.