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Purpose | Background |Procedure | Report

Paper Chromatography: A M&M’s® True Colors



EXPERIMENT NAME

CHEMISTRY HOMEPAGE
BLACKBOARD LOGIN
LAB MANUAL HOMEPAGE
SYLLABUS
 

 

The purpose should be a few sentences that cover the major concepts and techniques presented in this experiment. The procedure section should reference the lab manual and note any changes that were made to the procedure in the lab manual.

The data section for the experiment should contain the chromatogram that was created during lab, simply staple this to your report before you turn it in. Secondly the data section should include two data tables. The first data table should cntain data for each of the dye components found in the food colorings (each color may have several components, make sure you set up your table to show which color has which components) and their corresponding Rf values. The second table should include the dye components of each M&M color and their Rf values.

The Rf values can be determined using the following formula and a sample calculation of this should be included in the calculation section.

Rf Equation

The conclusion section should be several paragraphs addressing the following: a) the identity of the dye components of each of the M&M candy shells, b) a supported discussion of the identity of the dye components based on the food coloring Rf values (be sure to use Rf values to support your conclusions), c) a discussion of any dyes that do not appear to be any of the four food coloring colors tested (make sure to use the Rf values to support this discussion also), d) an explanation of why the components  moved up the paper at different rates and e) any errors in the experiment.

Answer the following Questions:

1) The presence of atoms from the far right side of the periodic table (not including the noble gases) in a molecule can make a molecule polar. Look up the definition of polarity and describe the dyes you have experimented with as polar or non-polar and explain your description.

2) TLC is a form of chromatography that can be used to determining the amino acid content of a protein mixture. Explain how this process might work.

 

 

 

 

 

 

 

 

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